Figure 6. Transient knockdown of cell cycle regulators influence the level of AA-induced cellular senescence.
Transient knockdown of Id1, p16, or p21 were performed via specific siRNA transfection 24 hours prior to AA (3 × 10−5 M) treatment and subsequent SA β-gal staining in LNCaP cells. The 4 × 200 cells were analyzed 3 days after siRNA transfection and treatment in the absence or presence of AA and plotted as a percentage of SA β-gal-positive cells. Scrambled siRNA served as control. Error bars indicate the SD of the mean of triplets.