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. 2014 Jul 29;28(9):1448–1459. doi: 10.1210/me.2014-1183

Figure 4.

Figure 4.

Effect of miRNAs on endogenous GHR mRNA and protein in HEK293 cells. A, Expression of human total GHR mRNA in HEK293 cells transfected with 50nM miR-129–5p, miR-142–3p, miR-202, and miR-16 mimics. The data are presented as mean ± SE of 3 individual experiments for all but miR-142–3p (n = 5); duplicates were assayed within each experiment. Statistically significant differences between the negative control and miR-treated cells were found for miR-129–5p, miR-142–3p, miR-202 (***, P ≤ .001) and miR-16 (*, P ≤ .05). B, Composite results for n = 4 individual experiments (mean ± SE) showing statistically significant differences between negative control and miR-treated cells: ***, P ≤ .001 for all treatments. C, Representative Western blot of GHR and calnexin protein levels in HEK293 cells after transfections with 50nM miR-129–5p, miR-142–3p, miR-202, and miR-16 mimics.