Figure 5.
EEF1A1, SSRP1, and XRCC6 exert ligand- and cell-specific effects on MR transactivation at the GILZ promoter. HEK293 and H9c2 cells transfected with GILZ-Luc and empty vector or EEF1A1 (panels A and B), SSRP1 (panels C and D), or XRCC6 (panels E and F) (50 or 200 ng) were treated with vehicle (white), aldosterone 10−8 M (gray) or cortisol 10−8 M (black) for 24 hours. Assays were performed twice in triplicate. Luciferase activity is expressed as relative to the hormone treated empty vector. Means were compared by ANOVA: *, P < .05; **, P < .01; ***, P < .001.