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. 2014 Jul 3;28(8):1329–1336. doi: 10.1210/me.2014-1042

Figure 1.

Figure 1.

Breeding scheme for androgenized zebrafish. Parent individuals (P) were crossed to generate embryos, which were split into 4 groups, exposed to nothing or to DME, T, or DHT at different doses (50, 500, and 1000 ng/L) during early or late time windows. The embryos were grown to the F0 zebrafish. The F0 individuals were then crossed with early androgen-exposed zebrafish or unexposed controls to generate 4 groups of F1 individuals: T500(F) × WT(M), T50 × T50, T1000 × T1000 and DHT50 × DHT50. Unexposed controls were included for both F0 and F1 generations.