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. 2014 May 30;28(7):1136–1149. doi: 10.1210/me.2014-1032

Figure 3.

Figure 3.

Increased apoptosis in mct8 morphants is not rescued by suppression of p53 signaling. A, Lateral (first row) and dorsal (second row) bright-field images of wild-type 25-hpf zebrafish noninjected embryos or embryos microinjected (1 nL) with CTRMO (0.6 pmol), MCT8MO (0.8 pmol), or MCT8+p53MO (0.8 + 0.5 pmol). The black box denotes the otic vesicle presented in a higher magnification in the insets. Scale bars, 100 μm. B, Total fluorescence (570 nm) measurement of noninjected and CTRMO, MCT8MO, or MCT8+p53MO microinjected wild-type 25-hpf zebrafish embryos after TUNEL using TMR-red. In the graph in B, bars denote SE, and experimental groups that do not differ significantly bear the same letter (P > .05) and those with different letters are significantly different (P < .0001) after Bonferroni multiple-comparison test.