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. 2014 May 30;28(7):1136–1149. doi: 10.1210/me.2014-1032

Figure 4.

Figure 4.

MCT8MO phenotype is rescued by MCT8 mRNA coinjection. A, Lateral (upper row) and dorsal (lower row) bright-field images of Tg(fli1:EGFP) 48-hpf zebrafish embryos noninjected and CTRMO (0.6 pmol), mutated mct8 mRNA (100 g), MCT8MO (0.8 pmol), or mutated mct8 mRNA+MCT8MO (100 pg + 0.8 pmol) microinjected (1 nL). Scale bars, 100 μm. B, Distribution of normal, morphant, and rescued animals in the different experimental groups according to axial trunk bending phenotype. C, Fluorescent images of central arteries invading the hindbrain of Tg(fli1:EGFP) 48-hpf zebrafish embryos noninjected and CTRMO, mutated mct8 mRNA, MCT8MO, or mutated mct8 mRNA+MCT8MO microinjected. The red chevron denotes midcerebral vein, red arrowheads denote central arteries invading the hindbrain, and cyan asterisk denotes otic capsule. Scale bars, 50 μm.