Skip to main content
. 2014 May 30;28(7):1136–1149. doi: 10.1210/me.2014-1032

Figure 7.

Figure 7.

At 31 (A–D) and 48 (E and F) hpf, mct8 morphants have fewer hindbrain and spinal cord neurons. A, Immunodetection of Zn8 in the hindbrain of 31-hpf wild-type zebrafish embryos microinjected with either CTRMO or MCT8MO. Arrowheads in A denote hindbrain interneurons, and red asterisks indicate trigeminal neurons. Scale bars, 50 μm. B, Expression of pax2a in 31-hpf wild-type zebrafish embryos microinjected with either CTRMO or MCT8MO. Black arrowheads indicate otic vesicle expression of pax2a. Scale bars, 100 μm. C, Immunodetection of acetylated β-tubulin in segments 4 to 10 of the spinal cord in 31-hpf wild-type zebrafish embryos microinjected with either CTRMO or MCT8MO. Scale bars, 50 μm. D, Expression of pax8 in 31-hpf wild-type zebrafish embryos microinjected with either CTRMO or MCT8MO. Arrows indicate first pax8-positive hindbrain interneurons. Scale bars, 50 μm. (e) Spinal cord expression of pax8 in 48-hpf wild-type zebrafish embryos microinjected with either CTRMO or MCT8MO. Scale bars, 200 μm. The boxed area in E corresponds to a higher-magnification image in F. Scale bars, 50 μm (F).