Figure 2.

Comparison of DHT-binding capacity assay and IFA values of recombinant wild-type and mutant SHBGs. Values for SHBG mutants in which amino acid substitutions introduced by nonsynonymous SNPs (■) are compared with a reference line generated using the values for wild-type SHBG (WT) determined at 5 different concentrations (♢). For reference, the corresponding values for a known DHT binding–deficient mutant, SHBG S42L (5), are shown (▵). Mutants suspected of exhibiting abnormal DHT-binding affinity or immune recognition in the IFA are circled and are positioned below or above the wild-type reference line, respectively.