Figure 3.
Osteoblasts are epigenetically engaged to become osteocytes. A, CEAS analysis of ChIP-seq histone levels in osteoblasts (d3, dashed lines) and osteocytes (d35, solid lines) for normalized gene profiles of genes up-regulated (red, ≥ 2 fold) or down-regulated (blue, ≤2-fold) during differentiation. Gene bodies were averaged to a 3-kb segment and displayed as an average gene. B, Overlaid ChIP-seq tag density profiles for histones around Sp7 and Pth1r (d3, red; d35, blue; overlap, purple), normalized to 107 tags. d3, day 3; d35, day 35; TSS, transcription start site.