Figure 4.

Osteocytes display a unique 1,25(OH)₂D₃-sensitive transcriptome. A, Heat map of regulated genes' expression in osteoblasts (d3) and osteocytes (d35) treated with vehicle (V) or 100 nM 1,25(OH)₂D₃ (D) for 24 hours. B, Venn diagram of 1,25(OH)₂D₃-regulated genes in the osteoblast (d3) and osteocyte (d35). C, The top GO term from the top 3 annotation clusters from DAVID are shown with associated P values. D, Gene expression (vehicle only) was normalized to β-actin levels and shown as fold change compared with d35 sample. Samples were analyzed in triplicate ± SEM. d3, day 3; d35, day 35.