Figure 8.

PRMT2 is involved in UV-induced DNA damage repair and homologous recombination. A, MCF-7 cells were transfected with control-siRNA or PRMT2-siRNA-1 for 24 hours. Genomic DNAs were collected at five different time points before or after UV treatment (25 mJ/cm²) and transferred to a nylon filter for Southwestern blot. Experiments were conducted twice independently in duplicate and representative data were shown. B, A schematic graph shows DR-GFP recombination reporter system. C, MCF-7 cells containing the DR-GFP plasmid were transfected with control-siRNA or PRMT2-siRNA-1 for 24 hours. Cells were transfected with the pCBAsce plasmid to generate DSB. GFP expression by homologous recombination was measured by a flow cytometry. Gene conversion was calculated by normalizing to control. The experiment was repeated three times independently.