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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 18;1862(6):1111–1121. doi: 10.1016/j.bbadis.2016.02.007

Figure 1. Alteration of hepatic connexin protein expression after APAP overdosing.

Figure 1

Mice (n = 5) were injected with 300 mg/kg APAP or saline (SAL) followed by sampling at different time points (i.e. after 0, 1, 6 and 24 hours). Hepatic protein levels of (A) phosphorylated (P1-P2) and non-phosphorylated (NP) Cx43, (C) Cx32 and (E) Cx26 were assessed by immunoblot analysis and expressed as relative alteration compared to untreated animals. mRNA was extracted and subjected to RT-qPCR analysis of (B) Cx43, (D) Cx32 and (F) Cx26. Relative alterations in mRNA levels were calculated according to the 2-ΔΔCq formula. Data are expressed as means ± SEM with *p ≤ 0.05 **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001 compared to saline at indicated time points.