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. 2014 Mar 14;28(5):634–643. doi: 10.1210/me.2013-1424

Figure 4.

Figure 4.

Examination of the internalization and β-arrestin-binding properties of N321K-V2R. HEK293 cells were transiently transfected with the plasmids of the indicated BRET partners and after 24 hours, the cells were exposed to AVP or vehicle (veh). A and B, β-Arrestin binding was measured with the transfection of WT- or N321K-V2R-mVenus and β-arrestin-Rluc plasmids. A, Cells were exposed either to 1 μM AVP (■) or vehicle (dashed line) at the indicated time points. B, Dose-response curve of β-arrestin-binding of AVP. The effect of the hormone on cells was calculated as the BRET ratio difference between the ligand (stim)–treated and the vehicle (nstim)–treated cells. C, Internalization kinetics was measured with the transfection of WT- or N321K-V2R-Sluc and MP-YFP plasmids. Cells were exposed to either 1 μM AVP or vehicle at the indicated time points. Mean values ± SE are shown (n = 3).