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. 2017 Mar 7;6(4):e1292195. doi: 10.1080/2162402X.2017.1292195

Figure 5.

Figure 5.

LYG1 promotes the activation, expansion, and IFNγ production of tumor antigen-specific CD4+ T cells. B6 mice were inoculated s.c. with irradiated B16-OVA cells. After 10 days, the splenocytes were isolated, stimulated with OVA323–339 in the presence of rhLYG1 (0, 1, 10, or 100 ng/mL) and then analyzed for activation, proliferation, and IFNγ production gated on CD4+ T cells by flow cytometry on day 3 or day 5. (A) The expression of CD69. The frequencies of CD69-expressing cells gated on CD4+ T cells are shown on the vertical axis on day 3 or day 5. (B) Proliferation was determined by the CFSE dilution assay. The percentages of CD4+ T cells that had divided at least once are indicated on day 3 or day 5. (C) The expression of IFNγ on day 5. The percentages of IFNγ+ cells gated on CD4+ T cells are shown on the vertical axis. (D) Naive CD4+ T cells from OT-II mice were stimulated with irradiated OVA323–339-pulsed DCs with the addition of the indicated concentration of rhLYG1. The expression of IFNγ at 24 h was measured by ELISPOT. One representative of three independent experiments was shown for each figure. Data are shown as mean ± SEM, *p < 0.05 and **p < 0.01 compared with medium (LYG1 not added).