Skip to main content
. 2017 Mar 7;6(4):e1292195. doi: 10.1080/2162402X.2017.1292195

Table 1.

Primers used in qPCR to examine mouse chemokines. qPCR was performed for quantitative analyses in an ABI Prism 7000 Sequence Detection System (Applied Biosystems). Amplifications were performed using Universal Probe Library (UPL) probes. The quantification data were analyzed with ABI Prism 7000 SDS software. The expression levels of the target genes were normalized to the internal standard gene GAPDH.

Chemokine Primers-forward (5′–3′) Primers-reverse (5′–3′)
CCL3 CCATGACACTCTGCAACCAA GTGGAATCTTCCGGCTGTAG
CCL4 GCCCTCTCTCTCCTCTTGCT GGAGGGTCAGAGCCCATT
CCL5 TGCAGAGGACTCTGAGACAGC GAGTGGTGTCCGAGCCATA
CCL8 TTCTTTGCCTGCTGCTCATA GCAGGTGACTGGAGCCTTAT
CXCL9 CTTTTCCTCTTGGGCATCAT GCATCGTGCATTCCTTATCA
CXCL10 GCTGCCGTCATTTTCTGC TCTCACTGGCCCGTCATC
CXCL11 TGCTGAGATGAACAGGAAGGT CGCCCCTGTTTGAACATAAG
GAPDH CACCAACTGCTTAGCCCCC TCTTCTGGGTGGCAGTGATG