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. 2017 May 3;12(5):e0176609. doi: 10.1371/journal.pone.0176609

Fig 1. Acute effects of 20 mM La DM incubation on p38 MAPK signaling in differentiating C2C12 myoblasts.

Fig 1

A Representative western blots of C2C12 cell lysates after up to 24 h of incubation with 0 or 20 mM La DM. B Quantification of WBs for p-p38 MAPK at T180 and Y182 (p-p38) revealed increased p-p38 following 0 mM La DM treatment for up to 4 h but unchanged levels in 20 mM La DM condition. C Quantification of H3K4 in the trimethylated state (H3K4me3) showed a pattern similar to p-p38 with a pronounced increase in control samples (0 mM La DM) within 4 h. Treatment with 20 mM La DM did not alter H3K4me3 levels within 4 h, but after 24 h H3K4me3 was not detectable. D Representative pictures of C2C12 cells after 15 min of incubation with 0 or 20 mM La DM followed by IF staining for H3K4me3 (green) and DAPI (blue). E Quantification of H3K4me3 staining intensity in C2C12 cells showed decreased levels already after 15 min in 20 mM La DM conditions compared to 0mM treated cells. * indicates statistical significance, i.e. p < 0.05.