Skip to main content
. 2017 May 3;12(5):e0176609. doi: 10.1371/journal.pone.0176609

Fig 3. La-induced delay in late differentiation in C2C12 cells is similar to p38 MAPK-inhibition in cells treated with 0 mM La DM + SB203580.

Fig 3

A Myogenin- (green, Alexa 488) and DAPI-staining (blue) in C2C12 myoblasts before (pre) and after 5 days of incubation with the respective DM. B Representative western blots for Pax7, Myf5, myogenin, and MHC. Actin was used as a loading control. C, E, G, I Ratio of positively stained nuclei in IF analysis. D, F, H, J Quantification of western blots for Pax7 (C, D), Myf5 (E, F), myogenin (G, H), and MHC (I, J). Data presented as mean ± SD. * indicates statistical significance, p < 0.05. For every marker analysed, differences between 0 mM La DM or 0 mM La DM + DMSO and 0 mM La DM + SB203580 or 20 mM La DM were significant (p<0.01).