FIGURE 3:

Rqc1 cosediments in light-sedimenting fractions in the absence of Rqc2, Ltn1, or Cdc48. Sedimentation profile (absorbance 260 nm) and Western blots performed with polysome extracts prepared from cells expressing Rqc1-TAP with a wild-type background, deleted for LTN1 or RQC2 or depleted for Cdc48, separated on 10–30% sucrose gradients. The localization of Rqc1-TAP was assessed using PAP antibodies; the 60S fractions and the Cdc48-sedimenting fractions were determined with anti-Nog1 and anti-Cdc48 antibodies, respectively.