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. 2017 May 1;28(9):1177–1185. doi: 10.1091/mbc.E16-11-0790

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© 2017 Marsboom et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Interaction between WT and mutant Cav1. (A) Cotransfection of WT Cav1 tagged with YFP and Cav1* tagged with Myc in Cav1⁻^/⁻ fibroblasts confirmed that mutant Cav1* colocalized with WT Cav1. Cells transfected with YFP served as a negative control, demonstrating the specificity of Myc staining. Scale bar, 20 μm. (B) Immunoprecipitation with antibody against YFP was able to pull down Myc-labeled Cav1*, demonstrating interaction of WT and mutant Cav1*. Nontransfected cells were used as controls to exclude nonspecific bands due to light or heavy chain contamination. PD YFP, pull down of wild type Cav1; PD Myc, pull down of Cav1*.