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. 2017 May 1;28(9):1258–1270. doi: 10.1091/mbc.E17-01-0020

FIGURE 2:

FIGURE 2:

Rac disruption does not rescue cytokinesis failure in formin(ts) embryos. (A, B) Kinetic analysis of contractile ring constriction in control and formin(ts) mutant one-cell embryos with and without Rac(G60R), dividing at (A) semirestrictive (23.5°C) and (B) semipermissive (18.5°C) temperature. Mean ring diameter vs. time, with all replicates for the formin(ts); Rac(G60R) double mutant in a more transparent shade of the same dark blue. Error bars represent SEM. The aqua arrow in A shows the more persistent attempt at contractile ring constriction seen in formin(ts) single-mutant embryos but not in formin(ts); Rac(G60R) double-mutant embryos. (C, D) Cytokinesis failure and success rates for different genotypes at (C) semirestrictive and (D) semipermissive temperature. The number of embryos per genotype is indicated on each individual bar; p values were obtained by both Fisher’s and Barnard’s exact tests (Supplemental Table S3). (E, F) Average peak rate of contractile ring constriction plotted as the change in diameter (µm/s) when the rate of ingression peaks from ∼90–50% ring constriction (or the point of maximum contractile ring constriction when embryos did not constrict to 50% of the initial cell diameter) at (E) semirestrictive and (F) semipermissive temperature. Error bars represent SD; p values were obtained by an unpaired, two-tailed Student’s t test (Supplemental Table S3). n.s., p ≥ 0.05; ****p < 0.0001.