TABLE 3.

Specific Primer Sequences, Product Sizes, and References for Targeted Genes and Reference Gene for qRT-PCR Analysis^a

Gene of Interest	Primer Direction	Primer Sequence	Product Size, bp	Reference Sequence
MMP-1	Forward	5′-CCA AAG TCT CCA AGG GTC AA-3′	83	NM_001171139.1
	Reverse	5′-CTG TCC TTC AGG TCC ATC AAA-3′
MMP-9	Forward	5′-TGC GAG TTT CCG TTC ATC TT-3′	117	NM_001082203.1
	Reverse	5′-GTA GAG CTT GTC CTT GTC GTA G-3′
MMP-13	Forward	5′-GGG ATT CCC AAG AGA GGT TAA-3′	100	NM_001082037.1
	Reverse	5′-TCA TAG CTC CAG ACT TGG TTT C-3′
TIMP-1	Forward	5′-ACT CCC ACA AAT CCC AGA A-3′	98	NM_001082232.2
	Reverse	5′-GGA ACC ACG AAA CTG CAA-3′
ADAM-TS5	Forward	5′-GTC ATC CAT CCT CAC CAG TAT C-3′	116	AF317415
	Reverse	5′-TCG TGG TAC ATC TAG CAA ACA G-3′
GAPDH	Forward	5′-GGT CGG AGT GAA CGG ATT T-3′	114	NM_001082253.1
	Reverse	5′-TGT AGT GGA GGT CAA TGA ATG G-3′

^aADAM-TS5, aggrecanase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP, matrix metalloproteinase; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TIMP-1, tissue inhibitor of metalloproteinase-1.