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. 2017 May 3;12(5):e0176501. doi: 10.1371/journal.pone.0176501

Fig 4. Apoptotic effects of PD on MDA-MB-231 and MCF-7 human breast cancer cells.

Fig 4

Cells were treated with the indicated concentrations of PD for 48 h, stained with Muse Annexin V & Dead Cell reagent and then analyzed for apoptosis by Muse Cell Analyzer. The results indicate the percentage of Annexin V-positive cells (apoptosis). All experiments were performed in duplicate and yielded similar results. (A) Original images of apoptosis in MDA-MB-231 cells. (B) The percentage of cells undergoing apoptotic cell death is presented as the mean ± SD from three separate experiments in MDA-MB-231 cells. *P<0.05, vs. the respective controls. (C) Original images of apoptosis in MCF-7 cells. (D) The percentage of cells undergoing apoptotic cell death is presented as the mean ± SD from three separate experiments in MCF-7 cells. *P<0.05, compared with the respective controls. PD, polydatin.