Figure 2.

Histological characterization of P. obesus kidney using hematoxylin and eosin staining. Histological section of non-diabetic P. obesus kidney, fed with ND, evidence normal glomerulus encircled by a Bowman’s space and blood capillary have standard diameters. Absence of cell accumulation at the interstitial space denotes that inflammatory process does not occur. Proximal and distal tubes are well organized. Sections P. obesus kidney observed in 3, 6 and 9 months after animals were fed with an HED (B, C and D respectively shown an increment on the blood capillary diameters and blood flow, and incipient inflammation which is evidenced by cell accumulation at the interstitium appearing between tubules (focal interstitial inflammation) leading to their disorganization and denaturation. Glomerular hypertrophy characterized by the absence of the Bowman’s space is observed at the 3rd month and evolves toward atrophy at the 6th month leading to the total degeneration of the glomerulus at the 9th month. Asterisks correspond to glomerulus, filled-circles to blood capillary, empty-circles to the interstitium between tubules, stars to proximal and distal tubes and arrows points to the Bowman’s space. Scale bar is 50 μm.