Figure 4.

Histological characterization of P. obesus kidney using Masson’s Trichrom staining. Histological section stained with Masson’s Trichrom in normal kidney (A) of P. obesus fed with a ND does not show any deposit of extracellular matrix evidencing the absence of interstitium fibrosis. On the contrary, sections from kidney of P. obesus fed with an HED for 3, 6 and 9 months (B, C and D respectively) show interstitial fibrosis, characterized by a loss of renal parenchyma and the occurrence of collagen fibers accumulation (arrows), and an increment of the number of vessels (rhombus). Scale bar is 50 μm.