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. 2017 May 3;12(5):e0175407. doi: 10.1371/journal.pone.0175407

Fig 3. MKK7 was a novel target gene for miR-125a-5p.

Fig 3

(A) Putative miR-125a-5p binding site predicted by miRanda database in 3’-UTR of human MKK7 gene (MAP2K7) (up). Transient luciferase reporter assays. Constructed the fragment of MKK7 3’-UTR or empty luciferase reporter vectors were transfected into HUVECs together with miR-125a-5p-mimic. Co-transfection after 48hr, a dual-luciferase reporter assay was performed. The luciferase activities were measured for all vectors and then normalized to the luciferase activity of Renila vector (***p<0.01) (down). Transfection of miR-125a-5p overexpression vector significantly reduced MKK7 mRNA (B) and protein (C) levels in HUVECs as compared with control vector. Using miR-125a-5p inhibitor remarkably enhanced mRNA and protein expression of MKK7 in HUVECs as compared with mock transfection (D and E). All results are representative of three independent experiments. **p<0.05