Table 2. Sequence data obtained from sequencing for seven housekeeping genes of Paenibacillus larvae in this study.
Loci | Sites of analysis (bp)a | Sb | Hapb | Hdb | G+C content |
πb | θb | kb | Neutrality test | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|
TajimaDc | FuLiDc | FuLiFc | FuFsc | |||||||||
ilvD1 | 486 | 3 | 2 | 0.153 | 0.5077 | 0.00095 | 0.00148 | 0.4595 | -0.8019 | 0.9246 | 0.4847 | 1.592 |
PurH | 355 | 5 | 4 | 0.162 | 0.4805 | 0.00078 | 0.00341 | 0.2778 | -2.0071* | -3.4154** | -3.4863** | -2.433 |
Tri | 437 | 3 | 2 | 0.149 | 0.4744 | 0.00103 | 0.00164 | 0.4481 | -0.8225 | 0.9215 | 0.4738 | 1.557 |
recF | 460 | 6 | 5 | 0.158 | 0.4407 | 0.00094 | 0.0032 | 0.4234 | -1.9154* | -2.8568* | -3.0006* | -1.428 |
pyrE | 565 | 2 | 2 | 0.153 | 0.4829 | 0.00054 | 0.00085 | 0.3063 | -0.6955 | 0.7802 | 0.4111 | 0.859 |
sucC | 570 | 4 | 4 | 0.289 | 0.4477 | 0.00095 | 0.00167 | 0.542 | -1.0411 | -0.0235 | -0.3819 | -0.842 |
glpK | 675 | 5 | 6 | 0.328 | 0.493 | 0.00093 | 0.00175 | 0.6262 | -1.2043 | -0.725 | -1.0162 | -2.795 |
a Size of fragments after alignment and cutting.
b S, number of polymorphic sites; Hap, number of haplotypes; Hd, haplotype diversity; π, nucleotide diversity per site; θ, average number of nucleotide difference per site; k, average number of nucleotide differences.
c Statistical analysis of neutrality tests.TajimaD, Tajima’s D test; FuLiD, Fu and Li’s D test; FuLiF, Fu and Li’s F test; FuFs, Fu’s Fs test; Statistically significant was indicated by using
* (P <0.05) and
** (P < 0.02).