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. 2017 May 3;12(5):e0176166. doi: 10.1371/journal.pone.0176166

Fig 2. The BEVS successfully expressed secreted and correctly folded zebrafish RETecd, GFRα1 and GDNF.

Fig 2

(A) Purified zRETecd, zGFRα1 and zGDNF on a Coomassie stained gel at corresponding molecular weights of 95 kDa, 37 kDa and 20 kDa. (B) zGDNF monomer and dimer on a Coomassie stained gel, monomer seen in 1 mM DTT (+) treated sample and dimer seen without (-) DTT. (C) both zRETecd and zGDNF were sensitive to PNGase F treatment: zRET shifted from 95 kDa to 90 kDa and zGDNF from 20 kDa to 18 kDa on Coomassie stained gel.