Fig 3. Purified zRETecd exists as a dimer and monomer in solution.
(A) SEC separated affinity chromatography purified zRETecd as two populations of protein eluting as two peaks (peak 1 on the left and peak 2 on the right) as seen from the chromatogram. (B) Pooled and concentrated fractions of peak 1 and peak 2 run on BN-PAGE. The dimer eluted from 120 ml to 155 ml, migrating at 200 kDa, and monomer eluted at 156 ml to 180 ml, migrating at 95 kDa in BN-PAGE. (C) Elution fraction samples were collected from both peak 1 and peak 2 and the same fractions (numbered between the images) run on SDS-PAGE and BN-PAGE. For both peaks, a single 95 kDa band was seen in SDS-PAGE and two bands were seen in BN-PAGE.