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The anti-phosphotyrosine (pY) Western blotting reflects the level of phosphorylated tyrosine residues in RET (upper panels); anti-RET western blotting (lower panels) demonstrates equal loading in different samples. hGDNFIC, hGDNFBV and hGDNFEc produced hGDNF were used as controls at a range of concentrations stated in nM and media as a negative control.
