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. 2017 May 3;12(5):e0176166. doi: 10.1371/journal.pone.0176166

Fig 7. zGDNF supports the survival of cultured mammalian dopaminergic neurons.

Fig 7

(A) the counted number of embryonic dopaminergic neurons in the presence of zGDNF and hGDNFIC versus negative control (media), (B) immunofluorescent staining of midbrain culture on 5DIV with a marker for DA neurons: TH (red) and nuclear dye DAPI (blue) comparing 0.27 nM of hGDNFIC, zGDNF and no GFL (control). Black bars: zGDNF; red bars: hGDNFIC; white bar: negative control. N = 4 indiviaul experiments per condition; statistically different from control ***p< 0.0007, **p< 0.003, *p<0.03.