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. 2017 May 3;12(5):e0177198. doi: 10.1371/journal.pone.0177198

Fig 5. Knockdown of miR-21 increases the levels of PCAT29 and PDCD4.

Fig 5

(A, B) DU145 and LNCaP cells were transfected with a scrambled oligo sequence (anti-miR-scr) or anti-miR-21 (30 nM) for 24 h. Cells were then treated with vehicle or IL6 (10 ng/ml) for 24 h and the expression of PCAT29 were determined by real-time RT-PCR. IL-6 significantly decreased the expression of PCAT29 in the anti-miR-scr. Knockdown of miR-21 enhanced the expression of PCAT29 and abolished the effect of IL-6 in both cell lines. (C, D) DU145 and LNCaP cells were transfected with anti-miR-21 (30 nM) for 24 h and then treated with IL-6 (10 ng/ml) for another 24 h. Cell lysates were prepared and used for Western blotting analysis. IL-6 significantly decreased the expression of PDCD4. Knockdown of miR-21 increased basal PDCD4 levels and abolished the reduction of this protein by IL-6. (E, F) DU145 and LNCaP cells were transfected with siRNA against STAT3 (10 nM) for 24 h. Cells were then treated with IL-6 (10 ng/ml) for 24 h and lysates were used for Western blotting analysis. IL-6 reduced PDCD4 levels, but this effect was abolished by siSTAT3. Data are presented as the mean ± SEM of at least 3 independent experiments. Asterisks (*) and (**) indicate statistically significantly difference (p < 0.05) from scramble and from scramble +IL-6 treatment groups, respectively.