Fig 6. Resveratrol blocks IL-6 suppression of PDCD4 and PCAT29 and its induction of miR-21.

(A, B) Western blotting analysis of DU145 and LNCaP cells showed that IL-6 reduced the levels of PDCD4. Resveratrol (25 μM) treatment for 24 h blocked this reduction and increased the levels of PDCD4 above that of control. This effect of resveratrol persisted even in the presence of IL-6. (C, D) Resveratrol also increased PCAT29 by greater than 2-fold and this effect was partly reduced by concurrent IL-6 treatment. (E, F) Resveratrol restored IL-6 stimulated expression of miR-21 in LNCaP and DU145 cells. Data are presented as the mean ± SEM of at least 3 independent experiments. Asterisks (*), (**), and (***) indicate statistically significantly difference (p < 0.05) from vehicle, from vehicle+IL-6 and from resveratrol, respectively.