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. 2013 Jul 24;27(9):1429–1441. doi: 10.1210/me.2012-1351

Figure 5.

Figure 5.

Interaction of ERs with DNA in BELZ cell lines. A, ChIP experiments using immunoprecipitation (IP) of Flag (ERβ) and ERα on the ERE sites of the estrogen-responsive luciferase gene, after treatment of BELZ cell lines with ethanol (Ctrl) or E2 (10 nM) during 1 hour. Control immunoprecipitation was performed with beads alone (IP noAb). IP values represent percentage of inputs. Data represent the mean of 2 independent experiments ± SD. B, ChIP analysis after 2 immunoprecipitations (IP 1 and 2) using Flag and ERα antibodies in BELZ ERβ-2 cell lines after 1 hour of estrogen treatment (E2 10 nM) or not (Ctrl). IP values represent percentage of inputs.