Figure 7.

Effect of ERα and ERβ on RIP140 expression. A, BELZ and BELZ ERβ-2 cell lines were transiently transfected with the human RIP140 promoter reporter plasmids (25 ng). After 24 hours, cells were treated with E2. The luciferase activity in BELZ cell lines (Ctrl and E2) was normalized at 1. B, ChIP analysis with Flag or ERα immunoprecipitation on the ERE site of RIP140 promoter after treatment with ethanol (Ctrl) or E2 (10 nM) during 1 hour of BELZ and BELZ ERβ-2 cell lines. Immunoprecipitation with no antibody was used for negative control (IP noAb). IP values represent percentage of inputs.