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. 2013 Jul 24;27(9):1429–1441. doi: 10.1210/me.2012-1351

Figure 9.

Figure 9.

Knockdown of RIP140 expression in BELZ ERβ-2 cells. A, BELZ ERβ-2 cells were transfected with either small interfering control (siControl) or small interfering RIP140 (siRIP140) as indicated in Materials and Methods, and RIP140 mRNA expression was assessed by real time PCR. B, BELZ ERβ-2 cells were transfected with either siControl or siRIP140 or untransfected (w/o siRNA). After 24 hours, cells were treated with vehicle alone (EtOH), with 10−8 M E2 or PPT or with 10−9 M 8β-VE2. Luciferase activity was quantified as described in Materials and Methods. Results were expressed relative to control response set at 1.