Table 1.
Primers Used for Real-Time PCR Analysis
| Gene | Forward Primer | Reverse Primer | GenBank Accession Number |
|---|---|---|---|
| Adrb3 | GGCAACCTGCTGGTAATCAT | GCATTACGAGGAGTCCCAC | NM_013462.3 |
| AgRP | AAGCTTTGGCGGAGGTGCTAGAT | AAGCAGGACTCGTGCAGCCTTACA | NM_007427.2 |
| AVP | TCAACACTACGCTCTCCGCTT | CCTTTGCCGCCCGG | NM_009732.1 |
| CRFR1 | TGCCAGGAGATTCTCAACGAA | AAAGCCGAGATGAGGTTCCAG | NM_007762.4 |
| DIO2 | TCCTCCTAGATGCCTACAAACAGG | ATTCAGGATTGGAGACGTGCAC | NM_010050.2 |
| GHSR | AAACAGACAGTGAAGATGCTTGCTG | GGCTCGAAAGACTTGGAAAACAG | NM_177330.4 |
| HPRT1 | GCAGTACAGCCCCAAAATGG | GGTCCTTTTCACCAGCAAGCT | NM_013556 |
| Leptin R | ACACTGAAGGGAAGACACTG | GGGTTCTTAGGTAATGGCTCC | NM_146146 |
| MC3R | CTGTAGCAACGGGTGTCGG | ATCAGCCTGCCTCATCCC | NM_008561.3 |
| MC4R | CAAGAACCTGCACTCACCCA | GACCCATTCGAAACGCTCAC | NM_016977.3 |
| NPY | TCAGACCTCTTAATGAAGGAAAGCA | ATGAGGGTGGAAACTTGGAAAAG | NM_023456 |
| POMC | TGGCCCTCCTGCTTCAGA | GGATGCAAGCCAGCAGGTT | NM_008895 |
| SF-1 | CCAGACCTTTATCTCCATTGTCG | AGTGTCATCTGGTCAGCCACCT | NM_139051.3 |
| TRH | ACCTTGGTGCTGCCTTAGATTC | ATCTCCCCTCTCTTCGGCTTC | NM_009426.2 |
Abbreviations: HPRT1, hypoxanthine guanine phosphoribosyl transferase 1; Leptin R, leptin receptor; SF-1, steroidogenic factor 1.