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KISS1R heterozygous mutation found in an IHH patient. DNA was extracted from blood lymphocytes. The KISS1R exons were sequenced as explained in Materials and Methods. A, Chromatograms showing double peaks after nucleotide 1023. Inserted nucleotides are indicated in bold and underlined. B, Protein sequence of KISS1R showing the 3 PRR repeats and the additional PRR in bold.
