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Ala-KISS1R-mutants exert a dominant negative effect on the WT-KISS1R cell surface expression except the (PRR)3AAA mutant. HeLa cells were transfected with 1.5 μg of HA-KISS1R and 1.5 μg of nontagged KISS1R mutants. ELISA was performed as previously described. Data are reported as the mean ± SEM of 2 experiments performed in triplicates; *P < .5 and **P < .01.
