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. 2017 Mar 8;5:22–30. doi: 10.1016/j.omtm.2017.02.003

Figure 1.

Figure 1

Comparison of the Effect of Vectofusin-1 versus That of Retronectin on Transduction of VSV-G LVs in CD34+ HSPCs

(A) CB-derived human CD34+ cells were transduced with purified VSV-G-pseudotyped SIN-GFP LVs at MOI 160 (two hits at MOI 80). GFP expression was analyzed 3 and 5 days post transduction (p.t.) by FACS analysis. Values are the mean ± SEM of n = 3 independent transductions. (B) Percentage of GFP+/primitive (CD34+CD90+CD133+) and early (CD34+CD90−CD133+) progenitor cells 3 days p.t. evaluated by FACS analysis. Values are the mean ± SEM of n = 3 independent transductions. (C) FACS analysis of GFP expression analyzed 3 and 5 days after transduction of BM-derived human CD34+ HSPCs transduced with VSV-G pseudotyped SIN-GFP LVs at MOI 200 (two hits at MOI 100). Values are the mean ± SEM of n = 4 independent transductions. (D) FACS analysis of GFP+ expression in primitive (CD34+CD90+CD133+) and early (CD34+CD90−CD133+) progenitor cells 3 days after transduction. Values are the mean ± SEM of n = 4 independent transductions. (E) Quantification of SIN-GFP VCN/cell calculated on liquid culture. Each sample was evaluated by Q-PCR in triplicate.