Skip to main content
. 2017 Mar 8;5:22–30. doi: 10.1016/j.omtm.2017.02.003

Figure 2.

Figure 2

Comparison of the Effect of Vectofusin-1 versus That of Retronectin on Transduction Efficiency of RD3-MolPack-GFP LV in CD34+ HSPCs

(A) CB-derived human CD34+ cells were transduced with unpurified RD114-TR-pseudotyped SIN-GFP LVs at MOI 8 (two hits at MOI 4), and GFP expression was analyzed 3 and 5 days after transduction by FACS analysis. Values are the mean ± SEM of n = 3 independent transductions. (B) Percentage of GFP+/primitive (CD34+CD133+CD90+) and early (CD34+CD133+CD90−) progenitor cells 3 days after transduction evaluated by FACS analysis. Values are the mean ± SEM of n = 3 independent transductions. (C) BM-derived human CD34+ cells were transduced with unpurified RD114-TR pseudotyped SIN-GFP LVs at MOI 8 (two hits at MOI 4), and GFP expression was analyzed 3 and 5 days after transduction by FACS analysis. Values are the mean ± SEM of n = 4 independent transductions. (D) Percentage of GFP+/primitive (CD34+CD90+CD133+) and early (CD34+CD90−CD133+) progenitor cells 3 days after transduction evaluated by FACS analysis. Values are the mean ± SEM of n = 4 independent transductions. (E) Quantification of SIN-GFP VCN/cell calculated on liquid culture. Each sample was evaluated by Q-PCR in triplicate.