Figure 4.

Transduction Efficiency and Memory Phenotype of T Cells Transduced with RD3-MolPack-GFP LVs in the Presence of Vectofusin-1

(A) T lymphocytes were transduced with RD114-TR-pseudotyped SIN-GFP LVs (MOI 1 and 0.1) in the presence of 12 μg/mL Vectofusin-1, and the transduction efficiency was analyzed after 7 and 14 days by FACS analysis. (B) SIN-GFP VCN/cell was quantified 14 days p.t. by Q-PCR. Values in (A) and (B) are the mean ± SEM of n = 7 (MOI 1) and n = 4 (MOI 0.1) independent transductions. (C) Relative frequency of CD3⁺CD8⁺ T_CM (CD45RA⁻CD62L⁺), T_SCM (CD45RA⁺CD62L⁺CD95⁺), T_EM (CD45RA⁻CD62L⁻), and T_EMRA (CD45RA⁺CD62L⁻) 1 day p.t. in mock-transduced cells versus RD114-TR-pseudotyped SIN-GFP LV-transduced cells (MOI 1) in the presence of 12 μg/mL Vectofusin-1. (D) Percentage of GFP⁺ cells among T_CM, T_SCM, T_EM, and T_EMRA subtypes. Values in (C) and (D) are the mean ± SEM of n = 3 independent transductions.