Figure 5.

Comparison of the Effect of Vectofusin-1 versus Different Enhancers in a Static Small- and Large-Scale Transduction Protocol Using RD3-MolPack-GFP LV in T Cells

(A) T cells were transduced with RD114-TR pseudotyped SIN-GFP LVs (MOI 1) in the absence (none) or in the presence of either soluble Vectofusin-1 (12 μg/mL), polybrene (8 μg/mL), protamine sulfate (4 μg/mL), or Retronectin-coated dishes (1.2 μg/cm²) (n = 3). GFP expression in CD3⁺ T cells was evaluated 7 and 14 days p.t. (B) Average VCN/cell, quantified by Q-PCR, was evaluated 14 days p.t. in T cells transduced at MOI 1. Transduction efficiency (C) and VCN (D) of T cells transduced with RD114-TR-pseudotyped SIN-GFP LVs (MOI = 1) in a 24-well plate or bag with Vectofusin-1 or Retronectin (n = 3).