Figure 2.

Binding of Fondaparinux, Arixtra, to AAV-2

(A–C) Assays were by inhibition of binding to heparin, measured by SPR (A and B), or (C) inhibition of HeLa cell transduction. (A) SPR sensorgrams of AAV-2 binding to surface heparin in competition with solution of heparin/fondaparinux. The AAV-2 concentration was 0.5 nM, and solution concentrations of heparin or fondaparinux were 1,000 nM; (B) SPR sensorgrams of AAV-2 binding to the heparin surface in different solution concentrations of fondaparinux are shown. (C) Transduction by AAV-2 vector is inhibited in a dose-dependent manner by fondaparinux. Cells were transduced with an AAV-2 mCherry-encoding vector in the presence of increasing concentrations of fondaparinux, with transduction assayed by fluorescence, measured by flow cytometry. Error bars show SD of triplicate measurements.