Table 1.
Differences between AAV-DJ and Parental Strains
| AAV-2 | AAV-8 | AAV-9 | |
|---|---|---|---|
| Complete Structure | |||
| Residues | 217–735 | 220–738 | 219–736 |
| Sequence identity (%) | 93 | 89 | 85 |
| RMSDall (Å) | 1.1 | 1.2 | 1.0 |
| RMSDCα (Å) | 0.8 | 1.0 | 0.8 |
| Maximal Cα difference (Å) | 7.7 | 11.0 | 8.2 |
| VR-Ia | |||
| Residues | 262–268 | 263–271 | 262–270b |
| Sequence identity (%) | 33 | 67 | 100b |
| RMSDall (Å) | 4.0 | 6.7 | 0.8b |
| RMSDCα (Å) | 3.6 | 6.3 | 0.8b |
| Maximal Cα difference (Å) | 7.7 | 11.0 | 1.1b |
| VR-IVa | |||
| Residues | 449–468 | 452–471b | 451–469 |
| Sequence identity (%) | 45 | 95b,c | 45 |
| RMSDall (Å) | 1.9 | 1.2b | 2.9 |
| RMSDCα (Å) | 2.0 | 1.0b | 3.3 |
| Maximal Cα difference (Å) | 6.4 | 2.8b | 8.2 |
| All Regions Except VR-I and VR-IVa | |||
| Sequence identity (%) | 95 | 89 | 86 |
| RMSDall (Å) | 1.0 | 0.9 | 0.9 |
| RMSDCα (Å) | 0.6 | 0.5 | 0.4 |
| Maximal Cα difference (Å) | 1.7 | 1.5 | 1.4 |
Statistics were calculated with Superpose.71
a
Variable regions (VRs) are defined according to structural differences between AAV serotypes.72
b
The natural serotype, locally closest to AAV-DJ.
c
VR-IV shares greatest sequence identity with AAV-8, but at least one residue (457) and perhaps as many as three residues (455–457) have been derived from the sequence of AAV-2.