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Chromosome Spread of HeLa Cells
Representative FISH image of a chromosome spread of one HeLa cell transfected with the four TALENS targeting the MYC and IGH genes. The chromosome indicated by the red arrow exhibits the t(8:14) translocation, as evidenced by colocalized MYC (red) and IGH (green) signals. Left: enlarged image of the metaphase chromosome containing the t(8:14) translocation. At least 100 metaphases were analyzed to detect one translocation.
