Figure 3.

Sensitivity of the PCR-Based Detection of Translocations

(A) Intensity of bands obtained by the PCR amplification of serial dilutions of the DNA obtained from HeLa cells transfected with the four TALENS recognizing the MYC and IGH regions. The amplicons were obtained with MYC F and IGH R primers. (B) The intensity of each band was related to the number of transfected cells from which we obtained the DNA used for PCR. The reliability of dilutions was calculated by amplifying the same samples with MYC F + R and applying the same method of quantification. Error bars represent the variation of band intensities obtained in one transfection experiment. The experiment was repeated twice, and representative graphs from one experiment are displayed.