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. 2017 Mar 8;5:43–50. doi: 10.1016/j.omtm.2017.03.001

Figure 4.

Figure 4

ENIT Can Be Used to Detect the Efficiency of Various Gene-Editing Tools and Targets

(A) PCR on DNA extracted from HeLa cells transfected with the four TALENS recognizing the MYC and 4q (FSHD) regions. The represented amplicons were obtained using the following primer pairs: lane 1, 4q F+R; lane 2, MYC F + R; lane 3, MYC F + 4q R. (B) PCR on DNA extracted from untransfected HeLa cells. The represented amplicons were obtained using the following primer pairs: lane 1, 4q F + R; lane 2, MYC F + R; lane 3, MYC F + 4q R. (C) PCR on DNA extracted from HeLa cells transfected with the two TALENS recognizing the MYC region and the CRISPR/Cas9 for the EZH2 gene region. The represented amplicons were obtained using the following primer pairs: lane 1, MYC F + R; lane 2, EZH2 F + R; lane 3, EZH2 F + MYC R. (D) PCR on DNA extracted from untransfected HeLa cells. The represented amplicons were obtained using the following primer pairs: lane 1, MYC F + R; lane 2, EZH2 F + R; lane 3, EZH2 F + MYC R.