Figure 4.

Expression of Human CD46 in Murine Cells Facilitates Infections, but Is Not Sufficient to Permit Replication of Enadenotucirev

(A) CT26 murine adenocarcinoma cells stably expressing human CD46 were infected with enadenotucirev variants expressing GFP under the control of either the CMV promoter (EnAd-CMV-GFP) or the replication-dependent splice acceptor (EnAd-SA-GFP). The cells were observed using fluorescent microscopy. CT26 parental cells were used as a negative control and permissive DLD human adenocarcinoma cells as a positive control. (B) Virus genome levels were measured 5 days post-infection by qPCR. Data represent mean values ± SD (n = 3). (C) CD46 transgenic mice were injected intravenously with 1 × 10¹¹ particles of enadenotucirev (EnAd, n = 5); replication-incompetent enadenotucirev (EnAdCJ132, n = 4); or saline (n = 3) in a small pilot study. Virus genome biodistribution by qPCR was performed 1 (n = 1) and 72 hr (EnAd, n = 4 and EnAdCJ132, n = 3) post-virus administration. BM = bone marrow. Data represent mean values ± SD (n = 3).