REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
Xenopus laevis oocytes	Nasco International	http://www.enasco.com
Chemicals, Peptides, and Recombinant Proteins
1,2-diphytanoyl-sn-glycero-3-phosphocholine	Avanti Polar Lipids	Cat. #850356C
Tetrapentylammonium chloride	Sigma Aldrich	Cat. #258962
Rubidium chloride	Sigma Aldrich	Cat. #215260
Deposited Data
TREK-2 Down conformation	Dong et al., 2015	PDB: 4XDJ
TREK-2 Up conformation	Dong et al., 2015	PDB: 4BW5
TREK-2 with norfluoxetine bound	Dong et al., 2015	PDB: 4XDK
TWIK-1	Miller and Long, 2012	PDB: 3UKM
TREK-1	http://www.thesgc.org/structures/4twk	PDB: 4TWK
Experimental Models: Organisms/Strains
Xenopus laevis oocytes	Nasco International	http://www.enasco.com
Recombinant DNA
Human TREK-2 (KCNK10)	Source Bioscience	IRATp970A05115D
Human TREK-1 (KCNK2)	Source Bioscience	IRATp970F06122D
Software and Algorithms
GROMACS 4.6	Hess et al., 2008	http://www.gromacs.org/
MDAnalysis	Michaud-Agrawal et al., 2011	http://www.mdanalysis.org/
UCSF Chimera Dock Prep Tool	Moustakas et al., 2006	https://www.cgl.ucsf.edu/chimera/docs/ContributedSoftware/dockprep/dockprep.html
CGenFF	Vanommeslaeghe et al., 2010	https://cgenff.paramchem.org/
CG2AT	Stansfeld and Sansom, 2011	http://memprotmd.bioch.ox.ac.uk/