The crystal structure of S. cerevisiae Nsa1ΔC. (A) Comparison of full-length Nsa1 and the C-terminal cleaved Nsa1 by SDS-PAGE (B) Overall structure of the Nsa1 WD40 domain shown in rainbow color. Two different views are shown, including a side and top view of the WD40 domain. The N and C-termini are labeled and they lie adjacent to one another within the first blade of the WD40 domain. The individual blades are colored as follows: Blade 1 (red), Blade 2 (orange), Blade 3 (yellow), Blade 4 (green), Blade 5 (cyan), Blade 6 (blue), and Blade 7 (purple). (C) Schematic illustration of the organization of Nsa1. The numbers correspond to S. cerevisiae Nsa1 and each blade is colored as in Fig 1B. See also Figure S1 and S2.